primary antibodies against dnmt1  (Santa Cruz Biotechnology)

Journal: Virology journal

Article Title: Vpr driving DNA methylation variation of CD4 + T cells in HIV-1 infection.

doi: 10.1186/s12985-024-02363-5

Figure Lengend Snippet: Fig. 5 The impact of Vpr on DNA methylation of host cell is verified by western blot. (A, B) The effect of Vpr on differentially methylated cytosines (DMCs) in the promoter (A) or gene-body (B) region of DNMTs. (C) The effect of Vpr on protein levels of DNMT1 and DNMT3A. (D, E) The effect of Vpr on DMCs in the promoter (A) or gene-body (B) region of CTCF and PAX5. (F) The effect of Vpr on protein levels of CTCF and PAX5.

Article Snippet: Primary antibodies against DNMT1 (24206-1-AP) and DNMT3A (20954-1-AP), were purchased from proteintech Group (Wuhan, China).

Techniques: DNA Methylation Assay, Western Blot, Methylation

Journal: FEBS Open Bio

Article Title: Epigenome‐wide DNA methylation analysis of myasthenia gravis

doi: 10.1002/2211-5463.13656

Figure Lengend Snippet: (A) Protein level of DNMT1 expression in HT22 cell line. (B) Analyses of the protein level of DNMT1 expression in HT22 cell line normalized to those of β‐actin for each sample. **** P < 0.0001 versus the sham group. n = 4. The data are presented as mean ± standard deviation (SD). A one‐way ANOVA followed by Fisher's least significant difference was used for statistics.

Article Snippet: Primary antibodies against DNMT1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Standard Deviation

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Homocysteine accelerates hepatocyte autophagy by upregulating TFEB via DNMT3b-mediated DNA hypomethylation

doi: 10.3724/abbs.2023060

Figure Lengend Snippet: The effect of DNMT3b on TFEB DNA methylation and autophagy (A) The difference in TFEB mRNA and protein levels in hepatocytes exposed to Hcy and DC-05, TF-3, and nanomycin A (NA), the corresponding inhibitors of DNMT1, DNMT3a and DNMT3b, respectively. (B) The binding level between DNMT and the TFEB promoter in hepatocytes was examined by ChIP assay. (C) DNMT3b mRNA and protein levels were measured by qRT-PCR and western blot analysis, respectively in hepatocytes with DNMT3b adenovirus infection. (D) DNMT3b levels were measured by qRT‒PCR and western blot analysis in hepatocytes infected with sh-DNMT3b adenovirus (sh-DNMT3b-1, sh-DNMT3b-2, and sh-DNMT3b-3). (E) Quantitative analysis of DNA methylation of the TFEB promoter in hepatocytes after DNMT3b overexpression or inhibition. (F) The effect of DNMT3b overexpression on TFEB level in hepatocytes. (G) The binding level of DNMT3b to the TFEB promoter was measured by ChIP assay in hepatocytes exposed to Ad-DNMT3b. (H) Detection of LC3BII/I and p62 protein levels after overexpression of DNMT3b. (I) Confocal images of mRFP-GFP-LC3 fluorescent puncta in hepatocytes after exposure to Ad-DNMT3b (scale bar: 5 μm). Data are presented as the mean±SD from three independent experiments, * P<0.05, ** P<0.01.

Article Snippet: Membranes were blocked with 5% defatted milk and then incubated with primary antibodies against DNMT1 (PA5-30581; Thermo Fisher Scientific), TFEB (ab267351; Abcam, Cambridge, USA), DNMT3a (ab2850; Abcam), DNMT3b (ab2851; Abcam), p62 (ab109012; Abcam), microtubule-associated protein 1 light chain 3 (LC3; ab192890; Abcam), and β-actin (ab8226; Abcam) respectively, followed by incubation with horseradish peroxidase (HRP)-labelled secondary antibody.

Techniques: DNA Methylation Assay, Binding Assay, Quantitative RT-PCR, Western Blot, Infection, Over Expression, Inhibition